Filtering 5031 reads with min max 217 bp: keep 4270 (84.87%)
Filtering 5635 reads with min max 217 bp: keep 3197 (56.73%)
152 dereplicated files where merged into file:
"_data/3_OTU_clustering/A_all_files_united.fasta"
Total number of sequences (not dereplicated): 534201
United sequences are dereplicated + size filtered into a total of 95693 unique sequences.
File prepared for OTU clustering: B_all_derep.fasta
Clustering reads from
"B_all_derep.fasta"
otu_radius_pct = 3
strand = plus
Chimeras discarded: 206
OTUs written: 1149 -> file "C_OTUs.fasta"
read renamed! ame as in "B_all_derep.fasta"
Reads remapped!
Subsetting OTUs with 0.1 % anundance; Keeping 13 OTUs
Error in file(file, ifelse(append, "a", "w")) :
cannot open the connection
In addition: Warning messages:
1: In dir.create(temp_foldername) :
cannot create dir '_data/6_haplotypes/_data/5_mapp/K01_116_BR2B_BF24_merged_cut_trunc_minmax_RC_maxee.txt_9.txt', reason 'No such file or directory'
2: In file(file, ifelse(append, "a", "w")) :
cannot open file '_data/6_haplotypes/_data/5_mapp/K01_116_BR2B_BF24_merged_cut_trunc_minmax_RC_maxee.txt_9.txt/OTU_13/OTU_13_tab.csv': No such file or directory
>
VascoElbrecht
commented
5 years ago
Making folders is wrong (?) for some reason