W-L / simION

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Playback with promethion bulk data #2

Open DanteV19 opened 1 week ago

DanteV19 commented 1 week ago

Thanks to your well-documented code, I could run playback within a container using the MinION bulk file from readfish. I would like to run playback with the PromethION bulk file instead too.

However, I could not run the playback using the PromethION bulk file with the current toml and I suspect the toml from: _/opt/ont/minknow/conf/package/sequencing/sequencing_MIN114_DNA_e8_2400K.toml is a toml specifically meant for MinION bulk file. However, I do not see any way on how to configure using another toml like sequencing_PRO114* for PromethION. So far, the playback seems to use sequencing_MIN114_DNA_e8_2_400K.toml by default.

Which adjustments should I make to be able to use the right toml file for the simulated PromethION device?

W-L commented 2 days ago

Hi Dante, The code in this project is quite specific to my setup. So I hard-coded the sequencing protocol that I use to run the playback into the script that launches the protocol. This is the file https://github.com/W-L/simION/blob/main/code/start_protocol_mod.py and the hard-coded line is 620. You could either change the protocol there or delete that line and see if the script detects the necessary protocol by itself. I just wanted to make sure that it always uses the same one, that's why I hardcoded it there.