Open MeiShu00 opened 3 years ago
@MeiShu00 Thanks for being interested in DeepRepeat.
--basecalled_path "workspace/pass/barcode01"
and --basecalled_path "workspace/pass/barcode02"
). You might also need to use "--f5i" for different index files (such as --f5i barcode1.f5index
and --f5i barcode1.f5index
). The starting point of the analysis is based on BAM files.@liuqianhn
Thank you for your quick response! However, i still have the following questions:
python DeepRepeat.py Detect
into the terminal:
Can this be ignored or are the packages version clashing with one another? I installed all packages by the Install DeepRepeat via conda instructions on the github page.
2.Can your trained models be used for data that are guppy basecalled and aligned to GRCh38 genome?
If i choose to train my own model, would there be an issue if my data is guppy basecalled? (Since training of model requires basecalling via albacore v2.3 )
Regarding --f5i, i understand that if they do not exist, f5.f5index will be created. Does this mean that i do not need to generate them myself ? if so, do i still need to call this flag when running it in the terminal?
Thank you !
@MeiShu00
--f5i
generally. But since you have different barcode folders, it is better to generate index files for each folder (otherwise, the default path for fast5 files is directly under workspace/pass/ folder.@liuqianhn
Regarding point 4 in the previous comment, what tools would be recommended for generating index files for fast5 files in each folder ?
Thank you !
@MeiShu00 sorry that I forgot to reply this message. DeepRepeat/bin/scripts/IndexF5files can be use to build index files.
Hi,
I would like to seek your help in using DeepRepeat for my nanopore data. After installation i had this warning after entering python DeepRepeat.py Detect:
I also have the following questions:
Thank you !