The 4DN + BINA Tiered System of Microscopy Metadata Guidelines was developed by the 4DN Imaging Standards Working Group in collaboration with the BINA Data Management and Quality Control Working Group (https://www.bioimagingna.org/qc-dm-wg) for documenting microscopy experiments and assessing the quality of image data.
[x] I wish that the “Description” field was optional as I do not always have something useful to say that isn’t described elsewhere
Tier 1
[x] Under Objectives, there is a typo “Coverslip Thickness”
[ ] Tube lens – Add a new component termed "TubeLens" in the "Additional Optics" category.
"The tube lens is part of the “infinity-corrected optics” system for modern microscopes. The image distance beyond the objective lens is set to infinity, rather than a fixed focal length, so a tube lens is required to focus the parallel light rays to produce the intermediate image for the camera. In Nikon microscopes, the tube lens is inside the microscope base, between the objective and the camera. (Filter cubes are usually placed in infinity space). The magnification can be increased from 1X to 1.5X by using a tube lens with a longer focal length (from the default 200mm to 300mm)."
[x] Objective/CoverslipThickness --> Move this field to ObjectiveSettings to allow to record the actual coverslip thickness used
"Some of our objectives have a correction collar that adjusts for spherical aberration due to different glass thicknesses. On the lens, the coverslip thickness is listed as a variable. The coverslip thickness field only allows a single number."
[x] Objective/Correction Collar - add the following attributes
Objective/CorrectionCollar
Objective/CorrectionCollarType
ObjectiveSettings/CorrectionCollarPosition
"There are also correction collars marketed for various sources of spherical aberration, so lenses are marked as having a correction collar to adjust for a) temperature, b) immersion media, and c) coverslip thickness. I am not sure where to describe this feature."
Tier 2
[ ] Filter wheel – Add "FilterSet", "FilterWheel" and "FilterBlock" and organize as follows:
FilterBlock IsA FilterSet
FilterWheel IsA FilterSet
"For some of our microscopes, we have a filter wheel in the excitation and/or emission light path where the individual filter elements may be used in combination with multiple filter sets (example for FRET: Green (Ex1 Dichroic 1 Em1) Red (Ex2 Dichroic 1 Em2) Green-Red FRET (Ex1 Dichroic 1 Em2)). It would be great to be able to represent filter wheels somehow in the micro-meta diagram.
[x] "TransmittanceRange/ReflectanceRange" --> allow CutOut to be optional to allow for Longpass filters
strambc
commented
4 years ago 
General
Tier 1
[x] Under Objectives, there is a typo “Coverslip Thickness”
[ ] Tube lens – Add a new component termed "TubeLens" in the "Additional Optics" category.
"The tube lens is part of the “infinity-corrected optics” system for modern microscopes. The image distance beyond the objective lens is set to infinity, rather than a fixed focal length, so a tube lens is required to focus the parallel light rays to produce the intermediate image for the camera. In Nikon microscopes, the tube lens is inside the microscope base, between the objective and the camera. (Filter cubes are usually placed in infinity space). The magnification can be increased from 1X to 1.5X by using a tube lens with a longer focal length (from the default 200mm to 300mm)."
"Some of our objectives have a correction collar that adjusts for spherical aberration due to different glass thicknesses. On the lens, the coverslip thickness is listed as a variable. The coverslip thickness field only allows a single number."
"There are also correction collars marketed for various sources of spherical aberration, so lenses are marked as having a correction collar to adjust for a) temperature, b) immersion media, and c) coverslip thickness. I am not sure where to describe this feature."
Tier 2
"For some of our microscopes, we have a filter wheel in the excitation and/or emission light path where the individual filter elements may be used in combination with multiple filter sets (example for FRET: Green (Ex1 Dichroic 1 Em1) Red (Ex2 Dichroic 1 Em2) Green-Red FRET (Ex1 Dichroic 1 Em2)). It would be great to be able to represent filter wheels somehow in the micro-meta diagram.