search

WU-BIMAC / NBOMicroscopyMetadataSpecs

The 4DN + BINA Tiered System of Microscopy Metadata Guidelines was developed by the 4DN Imaging Standards Working Group in collaboration with the BINA Data Management and Quality Control Working Group (https://www.bioimagingna.org/qc-dm-wg) for documenting microscopy experiments and assessing the quality of image data.
GNU Lesser General Public License v3.0
15 stars 4 forks source link

STRAMBIO -- Quantum Efficiency should depend upon WL #61

Open strambc opened 2 years ago

strambc commented 2 years ago

Element name(s): Detector

Field name(s): QuantumEfficiency

Justification for the addition (brief and compelling): Often the QE of a detector changes depending on WL range. Therefore having a single QE is not meaningful.

Full description of the proposed addition (enough detail to implement): QE should be associated with the WL module

strambc commented 2 years ago

Allan Mullan, Andor, a.mullan@andor.com

The QE response depends on the wavelength being detected- therefore you would need to link QE to emitted wavelength. With CCD models more often than sCMOS you may have some sensor variants and options to account for also. Linked to QE and not mentioned is the role of the Camera window – these also have a range in response depending on the wavelength. For visible range work it is usually fairly consistent but once off middle of the visible and if you are doing anything towards UV or NIR you may choose another window and you can change the effective QE of the camera quite a lot by this.