WatsonLab / MAGpy

Snakemake pipeline for downstream analysis of metagenome-assembled genomes (MAGs) (pronounced mag-pie)
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Question about databases #27

Closed smb20200615 closed 3 years ago

smb20200615 commented 3 years ago

Hello,

I have been testing out your tool with my bacterial MAGs. Most of the diamond hits get assigned to archaea. I used the 10 minute install guideline for setting up the databases. Do you have any idea on why this could be happening? I am new to your work and don't know where to start with the troubleshooting.

Thank you and happy new year!

mw55309 commented 3 years ago

Are you using uniprot swiss-prot or uniprot TREMBL?

Could you post an example of a FASTA sequence?

mw55309 commented 3 years ago

Hey

There is a new release today that fixes a lot of sourmash and phylophlan bugs

Please test it!

Cheers Mick