To do: Weekly Goals

[MathewGolding]

08/01/24

• [x] Get a copy of mestrenova
• [x] Finish Batch 3 analysis
• [x] Prepare LCMS experiments
• [x] Revise ANOVA and t-tests (small break on DoE for now)
• [x] Test Excel/Bar code scanner for tracking compound/logistics

[Waztom]

@MathewGolding is the action not rather testing Excel/bar code scanner for tracking compound/logistics?

[MathewGolding]

I will add that on there now.

[MathewGolding]

15/01/24

• [ ] Get one windows "diamonised" machine in the RCaH - may go on for over 1 week
• [x] Get Dani to add his "hit v no hit" document from xchem to the master analysis sheet - can find very high affinity binders compared with my LCMS doc. - MATCH CONVERSION DATA WITH XRAY DATA
• [x] Run smaller scale LCMS (14 samples) - quite urgent: see if we can use IS with SM to extract conversion from crudes

2A - Should be receiving compounds soonish - Suzuki first -> reductive aminations, williamson ethers, amidations, etc. then Buchwald Hartwig.Prepare

• [x] Add 96 reactant vials plate to CAR and OT
• [x] COSHH assess incoming compounds
• [ ] CAR uploads
• [ ] Test bar code method scanning methodology with upcoming 2A run

[MathewGolding]

25/01/24

• [x] Begin analysis of LCMS data - (reactants and products, RRF values)
• [ ] Clean OT - canned air clean the track/rail
• [ ] Test Dry run/Wet run of 96 start mat vials - not urgent but to do
• [ ] Buy BB from the eyeballing of compounds recently done
• [ ] Car uploads
• [ ] Test bar code method scanning methodology with upcoming 2A run (overarking goal)
• [ ] Get one windows "diamonised" machine in the RCaH - may go on for over 1 week
• [x] Create project swim lanes!

[MathewGolding]

29/01/24

• [x] Look in cheminv to check whether the ring I liked exists on compounds we presently have
• [ ] Begin constructing slides for presentation - NCS1 and CAR Development
• [x] Sort ChemInv
• [x] Finish conversion analysis via lcms of the 14 sample set
• [x] Check for and prepopulate probation period documentation
• [x] Create ticket for CAS Number to SMILES search with PubChemPy
• [ ] Create project to do list tickets

[MathewGolding]

12/02/24

Science

• [x] Review talk outline in powerpoint for presentation, figures, etc

• [ ] Do paladium catalyst - solvent tests n.b. get Tom Webb's data (message today (12/02/24)) Don't have enough catalyst to reasonably do this for suzuki. Keep in mind for Buchwal-Hartwig. -> Research literature for the solubility of these catalysts.

• [x] Redo LCMS of starting materials in one LCMS plate well (create mixture) - compare rrf value of at least one

• [x] Do test reactions on small scale suzuki test reactions to get used to them -> start with suz-2 4x4 combichem (analysis can also be done to do lcms for mscheck analysis)

  Lab operations

• [x] Create a cheminv catalogue ticket -> better organise the many tasks chem inventory needs doing (move old assignments to new - create physical and data names for the cabinets)

• [x] Get label maker working for the physical labels

• [x] Fill out waste disposal forms and clear out waste cabinet

• [x] Organise the "trolley" - select whether keep/disgard items

• [x] Dry and then wet run of 96 reactant vials

• [x] Update LCMS and CAR SOPs

• [ ] Create confluence page for ChemInv and other documentation

  Admin

• [ ] Create project to do lists to separate project goals and view them more succinctly:

  • [x] Create project tickets
  • [ ] Populate the tickets

• [ ] Check pubchem api methods for Cas number -> smiles searching

• [x] Order 96 well filtration plates - need quote from Biotage -> hasn't replied yet

[MathewGolding]

26/02/24

Science

• [x] Review talk outline in powerpoint for presentation, figures, etc

• [ ] Do paladium catalyst - solvent tests n.b. get Tom Webb's data (message today (12/02/24)) Don't have enough catalyst to reasonably do this for suzuki. Keep in mind for Buchwal-Hartwig. -> Research literature for the solubility of these catalysts.

• [x] Review Tomm's uploads to CAR (@Waztom to share) for Suz-2 reactions

• [ ] Add a multi-suzuki recipe to CAR and test upload/script creation

  Lab operations

• [x] Waste cabinet organisation - NOTE: get caps for waste bottles

• [ ] Organise the "trolley" - NOTE: select whether keep/disgard items - go through after meeting

• [x] Update LCMS and CAR SOPs - NOTE: Update encoded recipe used

• [ ] Create confluence page for ChemInv and other documentation - @Waztom to create first fast forward fragments confluence page

• [x] LCMS of all start materials again

• [x] Measure accuracy and precision of OT 10 uL transfers (0.5-5 uL scale) and then 300 uL (20-200 uL scale)

  Admin

• [x] Create project to do lists to separate project goals and view them more succinctly:

  • [x] Create project tickets
  • [x] Populate the tickets

• [ ] Check pubchem api methods for Cas number -> smiles searching

• [x] Set up MestReNova

• [x] T-Server into the DIAMRD4323 machine

[MathewGolding]

08/04/24

• [ ] Contact starlab about getting a 96 well format for the pipette tips see #86 for more details
• [ ] Meeting next week on 15th - good learning opportunity for me to gain further insight into the stages of how a user project starts within XChem
• [ ] Pre-populate 6 months probation form to go over with @Waztom afterwards
• [ ] Organise Github channels
• [ ] Catch-up on Scarab (High priority)

[MathewGolding]

08/04/24

Science

• [x] Hand over plate XC-EVA712A-wasgo-iter-1.0-2 to Ryan for soaking
• [x] Concentrate and transfer to a new plate the remaining successful Wasgo iter-1-0 compounds to a LDV plate (4uL)
• [x] Wasgo iter-1 two step reactions - review CAR recipes and plan experimental protocol and prepare CAR upload CSV
• [ ] Wasgo iter-2 - review compounds ordered and plan chemistry to execute on CAR for iter-2 compounds - tbd next week
• [ ] Get press to lab - speak to Mic (beamline engineer)
• [ ] Plan a suitable set of reaction that would prove the effectiveness of "print-press" reactions in a HTE format - ticket - next week

  Lab Operations

• [x] Contact Charlie on how to create a confluence page of ChemInv see #94 for futher details
• [ ] Finalise SOP on lab maintance

  Admin

• [x] Scarab (High Priority) see #93 for futher details
• [x] Contact starlab - 96 tip rack for 200uL xl tips
• [x] Prepopulate 6 month probation form -> find out from HR if I need to do a performance development review (email and copy warren in)
• [x] CHIKVMAC-FFF meeting 15th Apr

[MathewGolding]

22/04/24

Science

• [x] Begin preperation and execute synthesis of Batch 2 of the two step iter 1.1 wasgo compounds
• [ ] Complete batch 1 LCMS analysis on Batch 1 of the two step iter 1.1 wasgo compounds - 0.1 uL pipette dilution and start batch 2 LCMS analysis
• [ ] Wasgo iter-2 - review compounds ordered and plan chemistry to execute on CAR for iter-2 compounds - tbd next week
• [ ] Get press to lab - speak to Mic (beamline engineer) - tbd next week
• [ ] Plan a suitable set of reaction that would prove the effectiveness of "print-press" reactions in a HTE format - ticket - tbd next week

  Lab Operations

• [x] Switch LCMS back on post trough installation (Tuesday)
• [x] Review and finalise wording of lab maintanance SOP checklist
• [ ] Confirm Disk space availble on LCMS machine (Both C: and D: drives) (High Priority, do immediately after LCMS turn on)

  Admin

• [x] Prepopulate 6 month probation form

[MathewGolding]

29/04/24

Science

• [x] Prepare 0.1 mM standard and run LCMS for wasgo iter 1.1 batch 1 (high priority - today)

• [ ] analyse batch 1 via MSCheck and manual review to gain conversion estimates

• [ ] Get press to lab - speak to Mic (beamline engineer) - tbd next week?

• [ ] Plan a suitable set of reaction that would prove the effectiveness of "print-press" reactions in a HTE format - ticket - tbd next week?

• [ ] Wasgo iter-2 - review compounds ordered and plan chemistry to execute on CAR for iter-2 compounds - tbd next week?

  Lab operations

• [ ] Begin fridge/freezer clean up?

• [x] Review CAR 384 plates 100 uL labcyte echo set up

Admin

• [ ] Reword probation form and prepopulate PDR

[MathewGolding]

08/05/24

Science

• [x] Prepare 0.1 mM standard and run LCMS for wasgo iter 1.1 batch 1 (high priority - today)

• [ ] analyse batch 1 via MSCheck and manual review to gain conversion estimates

• [ ] Get press to lab - speak to Mic (beamline engineer) - tbd next week?

• [ ] Plan a suitable set of reaction that would prove the effectiveness of "print-press" reactions in a HTE format - ticket - tbd next week?

• [ ] Wasgo iter-2 - review compounds ordered and plan chemistry to execute on CAR for iter-2 compounds - tbd next week?

  Lab operations

• [ ] Begin fridge/freezer clean up?

Admin

• [ ] Reword probation form and prepopulate PDR

[MathewGolding]

28/05/24

Science

• [ ] analyse iter 1.0 and iter 1.1 batch 2 via MSCheck excel template to gain conversion estimates (~100)

• [ ] Get Lab PC and network this PC to diamond (@Waztom)

• [ ] Get press to lab - speak to Mic (beamline engineer) - tbd next week?

• [ ] Plan a suitable set of reaction that would prove the effectiveness of "print-press" reactions in a HTE format - ticket - tbd next week?

• [ ] Wasgo iter-2 - review compounds ordered and plan chemistry to execute on CAR for iter-2 compounds - tbd next week?

  Lab operations

• [ ] Begin fridge/freezer clean up

• [ ] Begin Lab clean up -> see action list in emails from Warren

Admin

• [ ] Refine Scarab entries and input Scarab of Wasgo iter 1.0 and 1.1 to high quality level