I have encountered the follow error message with the dataset that I am currently trying to analyze and would like to ask if you have any idea what the source of the issue is and how it can be fix it.
Thanks a lot for your help.
Warning: no random primer length was specified, but at least one RNA is longer than a typical directed-primer amplicon. Use --random-primer-len to exclude mutations within primer binding regions.
Created pipeline at 2021-11-22 18:03:58
Running FastaFormatChecker at 2021-11-22 18:03:58 . . .
. . . done at 2021-11-22 18:03:59
Running BowtieIndexBuilder at 2021-11-22 18:03:59 . . .
. . . done at 2021-11-22 18:03:59
Running process group 3 at 2021-11-22 18:03:59 . . .
Including these components:
ProgressMonitor . . . started at 2021-11-22 18:03:59
QualityTrimmer1 . . . started at 2021-11-22 18:03:59
QualityTrimmer2 . . . started at 2021-11-22 18:03:59
Interleaver . . . started at 2021-11-22 18:03:59
Merger . . . started at 2021-11-22 18:03:59
LengthFilter . . . started at 2021-11-22 18:03:59
BowtieAligner . . . started at 2021-11-22 18:03:59
MutationParser_Modified . . . started at 2021-11-22 18:03:59
MutationCounter_Modified . . . started at 2021-11-22 18:03:59
Traceback (most recent call last):
File "/programs/x86_64-linux/shapemapper2/2.1.3/python/cli.py", line 108, in
run(sys.argv)
File "/programs/x86_64-linux/shapemapper2/2.1.3/python/cli.py", line 65, in run
success = pipeline.run()
File "/programs/x86_64-linux/shapemapper2/2.1.3/python/pyshapemap/pipeline.py", line 743, in run
s = self.get_error_message(run_group)
File "/programs/x86_64-linux/shapemapper2/2.1.3/python/pyshapemap/pipeline.py", line 590, in get_error_message
msg += c.get_error(verbose=verbose)
File "/programs/x86_64-linux/shapemapper2/2.1.3/python/pyshapemap/component.py", line 565, in get_error
s += "\n" + self.read_stderr()
File "/programs/x86_64-linux/shapemapper2/2.1.3/python/pyshapemap/component.py", line 469, in read_stderr
f = open(self.stderr.output_nodes[0].filename, 'rU')
FileNotFoundError: [Errno 2] No such file or directory: 'shapemapper_temp/XYZ/Modified/ProgressMonitor/XYZ_Modified_ProgressMonitor_stderr.txt'
Hello Shapemapper2 team,
I have encountered the follow error message with the dataset that I am currently trying to analyze and would like to ask if you have any idea what the source of the issue is and how it can be fix it. Thanks a lot for your help.
Warning: no random primer length was specified, but at least one RNA is longer than a typical directed-primer amplicon. Use --random-primer-len to exclude mutations within primer binding regions. Created pipeline at 2021-11-22 18:03:58 Running FastaFormatChecker at 2021-11-22 18:03:58 . . . . . . done at 2021-11-22 18:03:59 Running BowtieIndexBuilder at 2021-11-22 18:03:59 . . . . . . done at 2021-11-22 18:03:59 Running process group 3 at 2021-11-22 18:03:59 . . . Including these components: ProgressMonitor . . . started at 2021-11-22 18:03:59 QualityTrimmer1 . . . started at 2021-11-22 18:03:59 QualityTrimmer2 . . . started at 2021-11-22 18:03:59 Interleaver . . . started at 2021-11-22 18:03:59 Merger . . . started at 2021-11-22 18:03:59 LengthFilter . . . started at 2021-11-22 18:03:59 BowtieAligner . . . started at 2021-11-22 18:03:59 MutationParser_Modified . . . started at 2021-11-22 18:03:59 MutationCounter_Modified . . . started at 2021-11-22 18:03:59 Traceback (most recent call last): File "/programs/x86_64-linux/shapemapper2/2.1.3/python/cli.py", line 108, in
run(sys.argv)
File "/programs/x86_64-linux/shapemapper2/2.1.3/python/cli.py", line 65, in run
success = pipeline.run()
File "/programs/x86_64-linux/shapemapper2/2.1.3/python/pyshapemap/pipeline.py", line 743, in run
s = self.get_error_message(run_group)
File "/programs/x86_64-linux/shapemapper2/2.1.3/python/pyshapemap/pipeline.py", line 590, in get_error_message
msg += c.get_error(verbose=verbose)
File "/programs/x86_64-linux/shapemapper2/2.1.3/python/pyshapemap/component.py", line 565, in get_error
s += "\n" + self.read_stderr()
File "/programs/x86_64-linux/shapemapper2/2.1.3/python/pyshapemap/component.py", line 469, in read_stderr
f = open(self.stderr.output_nodes[0].filename, 'rU')
FileNotFoundError: [Errno 2] No such file or directory: 'shapemapper_temp/XYZ/Modified/ProgressMonitor/XYZ_Modified_ProgressMonitor_stderr.txt'