WormLabCaltech / alaska

Automated and friendly RNA-seq analysis (deprecated)
http://alaska.caltech.edu
MIT License
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Life-stages to choose from #20

Open dangeles opened 5 years ago

dangeles commented 5 years ago

Life-stages to choose from in the metadata should include:

**** indicate that Raymond should approve these, since some of these life-stages may have different annotations in wormbase.

Single-cell Embryo 2-cell Embryo 4-cell Embryo * Embryo L1 L1 arrest L2 L2d Dauer L3 L4 Young Adult Adult Post-egg-laying adult (unmated) ** Post-egg-laying adult (mated) Aged Adult (Animals >7 days old)

Lioscro commented 5 years ago

@raymond91125 Are these the correct terms for life stage?

raymond91105 commented 5 years ago

Single-cell Embryo -> 1-cell embryo Ce 2-cell Embryo -> 2-cell embryo Ce 4-cell Embryo -> 4-cell embryo Ce Post-egg-laying adult (unmated) -> post-reproductive stage adult hermaphrodite Ce Post-egg-laying adult (mated) -> post-reproductive stage adult hermaphrodite Ce Aged Adult (Animals >7 days old) -> 7-days post-L4 adult hermaphrodite Ce

Life stage look up here https://wormbase.org/species/all/life_stage/WBls:0000671#02--10

Lioscro commented 5 years ago

@raymond91105 I've been browsing through the life stage link that you posted earlier, and I only see life-stage ontologies for C elegans and Strongyloides. Is there anywhere to get the terms for the other Wormbase organisms, or are those two the only ones that have been defined?

Lioscro commented 5 years ago

Also working on this in branch dev-life-stages.

raymond91125 commented 5 years ago

Likely any species-specific life stage should a subtype of the corresponding general term. When the species-specific ones don't conform to standard nomenclature, we then define new ones. Noted that the current ontology may be overloaded unnecessarily. (I haven't surveyed.) Perhaps provide a way for the user to submit new ones (for curator consideration) when they are needed.

Lioscro commented 5 years ago

Here are the ones I have: '1-cell embryo', '2-cell embryo', '4-cell embryo', 'Embryo', 'L1', 'L1 arrest', 'L2', 'L2d', 'Dauer', 'L3', 'L4', 'Young Adult', 'Adult', 'Post-reproductive stage adult', '7-days post-L4 adult'