Closed aprabhu90 closed 2 years ago
Hi Apoorva,
To get auxiliary scores you need to include the VIRSorter affi_contigs file. Without it we can't calculate the auxiliary score which we use to measure how confident a gene on a viral contig is actually viral and not a host gene erroneously included on the contig.
Mike
Hi,
I ran the latest dramv on putative vMAGs obtained from both virsorter2 and vibrant, followed by dereplication at 0.99 with CD-HIT to get putative vMAGs. I was hoping to include vMAGs identified by Vibrant as well through this pipeline.
The latest dram(dramv_1.2.3) ran smoothly on putative vMAGs without a hitch but I seem to have issues with running distill. I tried on a small subset of samples with a single annotation file
DRAM-v.py distill -i annotations.tsv -o Dramv_test
and obtained the following error message -Could this be a database issue as I can't seem to find anything close to "auxiliary score" column in the annotate output ? Have pasted below the annotation output if it helps.
Could you advise what is the alternate option for vMAGs obtained this way? Thanks, Apoorva