I followed the steps to download all the reference files to run, the first half showed that everything was fine, but there was an error in the STAR-fusion step.
Specific errors:Bio.Application.ApplicationError: Non-zero return code 2 from 'STAR-Fusion --left_fq /home/resource_files/out/patient1/tmp/rna_1_qc.fastq --right_fq /home/resource_files/out/patient1/tmp/rna_2_qc.fastq --genome_lib_dir /home/resource_files/mutect2_resource/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play/ctat_genome_lib_build_dir/ --output_dir /home/resource_files/out/patient1/tmp/star_fusion_outdir --CPU 15', message "Can't locate DB_File.pm in @INC (you may need to install the DB_File module) (@INC contains: /home/seq2neo/biosoft/STAR-Fusion.v1.10.1/util/../PerlLib /opt/rh/devtoolset-7/root//usr/lib64/perl5/vendor_perl /opt/rh/devtoolset-7/root/usr/lib/perl5 /opt/rh/devtoolset-7/root//usr/share/perl5/vendor_perl /home/seq2neo/miniconda3/envs/Seq2Neo/lib/site_perl/5.26.2/x86_64-linux-thread-multi /home/seq2neo/miniconda3/envs/Seq2Neo/lib/site_perl/5.26.2 /home/seq2neo/miniconda3/envs/Seq2Neo/lib/5.26.2/x86_64-linux-thread-multi /home/seq2neo/miniconda3/envs/Seq2Neo/lib/5.26.2 .) at /home/seq2neo/biosoft/STAR-Fusion.v1.10.1/util/../PerlLib/TiedHash.pm line 6."
LiJIAJUN-G
commented
11 months ago
I followed the steps to download all the reference files to run, the first half showed that everything was fine, but there was an error in the STAR-fusion step. Specific errors:Bio.Application.ApplicationError: Non-zero return code 2 from 'STAR-Fusion --left_fq /home/resource_files/out/patient1/tmp/rna_1_qc.fastq --right_fq /home/resource_files/out/patient1/tmp/rna_2_qc.fastq --genome_lib_dir /home/resource_files/mutect2_resource/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play/ctat_genome_lib_build_dir/ --output_dir /home/resource_files/out/patient1/tmp/star_fusion_outdir --CPU 15', message "Can't locate DB_File.pm in @INC (you may need to install the DB_File module) (@INC contains: /home/seq2neo/biosoft/STAR-Fusion.v1.10.1/util/../PerlLib /opt/rh/devtoolset-7/root//usr/lib64/perl5/vendor_perl /opt/rh/devtoolset-7/root/usr/lib/perl5 /opt/rh/devtoolset-7/root//usr/share/perl5/vendor_perl /home/seq2neo/miniconda3/envs/Seq2Neo/lib/site_perl/5.26.2/x86_64-linux-thread-multi /home/seq2neo/miniconda3/envs/Seq2Neo/lib/site_perl/5.26.2 /home/seq2neo/miniconda3/envs/Seq2Neo/lib/5.26.2/x86_64-linux-thread-multi /home/seq2neo/miniconda3/envs/Seq2Neo/lib/5.26.2 .) at /home/seq2neo/biosoft/STAR-Fusion.v1.10.1/util/../PerlLib/TiedHash.pm line 6."