Open auberginekenobi opened 2 years ago
Hi, thanks for your interest! The current complex SV assembling pipeline applies a set of relative stringent criteria to make sure every local assembly have smooth distance decay pattern of Hi-C contact frequencies across the breakpoint. And currently, the pipeline does not support the detection of circular DNA. But I do have plans to add this function in the next major version which might come out in the next a few months. I will keep you posted!
H Xiaotao, great tool filling an urgent unmet need.
I am running neoloopfinder on the following SVs:
As you may be able to tell, these breakpoints form a cycle, and indeed this is the location of an ecDNA in this sample. Therefore I would expect a connected assembly to be called by
assemble-complexSVs
:It appears that assemble-complexSVs filters the
chr1 chr1 -- 198993881 198984836 translocation
breakpoint and then fails to form any connected assembly.Furthermore, if I run
How would I go about applying Neoloopfinder to my ecDNA+ HiC data?
neoloop-caller
on theExpected.assemblies.txt
, the algorithm calls cis-loops within individual segments, but does not call any trans-loops or attribute any loops to the A0 assembly: