Xinglab / CLAM

CLIP-seq Analysis of Multi-mapped reads
GNU General Public License v3.0
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Question about permutation_callpeak #28

Open MarLee39 opened 1 year ago

MarLee39 commented 1 year ago

Hi,

Thank you for developing such a nice tool. I would really like to use CLAM in my project! I am a newbie in this field, and currently trying to analyze RIP-seq data.

I have a question about peak calling. I processed my data using multi-replicate mode though, I am not clear whether I should also try permutation_callpeak command. This command seems to process only one sample at a time according to your example, which means that I use this command when no multiple RIP-seq data is available? I have read your NAR paper, and struggled to understand the procedure, but it was a little bit difficult for me.

Hope you could help me for better understanding about the peak calling with CLAM.

Best,