Open xibeixingchen opened 2 years ago
From the stack trace, it looks like the segfault happened while reading the bam files on multiple threads: https://github.com/Xinglab/rmats-turbo/blob/v4.1.2/rMATS_pipeline/rmatspipeline/rmatspipeline.pyx#L987
My guess is that the segfault happens due to running out of memory. This thread has some suggestions: https://github.com/Xinglab/rmats-turbo/issues/18#issuecomment-763109643
Detailed errors were reported using gbd, when used code
python rmats.py --b1 /home/wengyy/work/mcl/BMK211105-AQ137-ZX01-0302/BMK_DATA_20211227144421_1/Customer_BMK211105-AQ137-ZX01-0302/result/as_out/CK4.txt --b2 /home/wengyy/work/mcl/BMK211105-AQ137-ZX01-0302/BMK_DATA_20211227144421_1/Customer_BMK211105-AQ137-ZX01-0302/result/as_out/AGE4.txt --gtf /home/wengyy/work/software/miniconda2/envs/rmats/rMATS/path/to/oat_gene_v2.gtf -t paired --readLength 100 --variable-read-length --nthread 8 --od /home/wengyy/work/mcl/BMK211105-AQ137-ZX01-0302/BMK_DATA_20211227144421_1/Customer_BMK211105-AQ137-ZX01-0302/result/as_out/output --tmp /home/wengyy/work/mcl/BMK211105-AQ137-ZX01-0302/BMK_DATA_20211227144421_1/Customer_BMK211105-AQ137-ZX01-0302/result/as_out/tmp_output
, Each of my BAM files is about 1GB in size, where one bam file was select on every group. How can I solve this problem?