XinweiSong
commented
4 weeks ago Thank you for your work. I think this is a very useful too for those interested in As research. I have been using this tool/database for annotating genomes of isolated genomes. I notice that there is discrepancy between AsgeneDB.fa headers and asgene.map.
I have applied filtering steps to ensure that if I hit a gene in the database that I can look at the asgene.map file to get a description.
Just wondering why the difference between the files.
Kind regards
Thank you for your kind words and for using our tool/database for annotating genomes. I'm glad to hear that it has been useful in your As research.
Regarding your observation about the discrepancy between the AsgeneDB.fa headers and the asgene.map file, I appreciate your attention to detail. The difference arises because we included some non-arsenic metabolism protein sequences in AsgeneDB.fa. This was done to reduce false positives that can occur during annotation with smaller databases. To ensure accuracy in annotation, the asgene.map file only contains the correct arsenic metabolism protein names and their corresponding gene names. This approach helps maintain the reliability of your annotations when you cross-reference with the asgene.map file.
Thank you for bringing this to our attention, and I hope this explanation clarifies the issue. Please don't hesitate to reach out if you have any further questions.
Kind regards
Thank you for your work. I think this is a very useful too for those interested in As research. I have been using this tool/database for annotating genomes of isolated genomes. I notice that there is discrepancy between AsgeneDB.fa headers and asgene.map.
I have applied filtering steps to ensure that if I hit a gene in the database that I can look at the asgene.map file to get a description.
Just wondering why the difference between the files.
Kind regards