YangLab / CIRCexplorer2

circular RNA analysis toolset
http://circexplorer2.readthedocs.org/
Other
76 stars 41 forks source link

Annotated 0 fusion junctions #6

Closed snehamurthy21 closed 7 years ago

snehamurthy21 commented 7 years ago

CIRCexplorer parameters: /usr/local/bin/CIRCexplorer2 annotate -r ref.txt -g ref.fa circ Start CIRCexplorer2 annotate at 11:44:56 Start to annotate fusion junctions... Annotated 0 fusion junctions! Start to fix fusion junctions... Fixed 0 fusion junctions! End CIRCexplorer2 annotate at 11:45:01

why would i get 0 fusion junctions could anyone suggest what to try?

kepbod commented 7 years ago

The expression of circRNAs is extremely low, so correct sequencing method and sufficient sequencing depth should be satisfied. Please make sure your datasets are correct.