Closed snehamurthy21 closed 7 years ago
CIRCexplorer parameters: /usr/local/bin/CIRCexplorer2 annotate -r ref.txt -g ref.fa circ Start CIRCexplorer2 annotate at 11:44:56 Start to annotate fusion junctions... Annotated 0 fusion junctions! Start to fix fusion junctions... Fixed 0 fusion junctions! End CIRCexplorer2 annotate at 11:45:01
why would i get 0 fusion junctions could anyone suggest what to try?
The expression of circRNAs is extremely low, so correct sequencing method and sufficient sequencing depth should be satisfied. Please make sure your datasets are correct.
CIRCexplorer parameters: /usr/local/bin/CIRCexplorer2 annotate -r ref.txt -g ref.fa circ Start CIRCexplorer2 annotate at 11:44:56 Start to annotate fusion junctions... Annotated 0 fusion junctions! Start to fix fusion junctions... Fixed 0 fusion junctions! End CIRCexplorer2 annotate at 11:45:01
why would i get 0 fusion junctions could anyone suggest what to try?