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YanqiangLi / NanoNm

MIT License
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questions about detect the Nm of mRNA by NanoNm if can use .bam data #3

Open violet-everygarden opened 1 week ago

violet-everygarden commented 1 week ago

Hello, we tried to use the NanoNm to detect the Nm site of directRNA-seq data, but the server can't run such a large amount of data. I would like to ask whether it is possible to use .bam data for Nm prediction? If so, how should the code be changed?

YanqiangLi commented 6 days ago

Hello there, I do not think the only .bam data is a valid input for NanoNm. I suggest you can extract the features for each fast5 folder, merge all of these features together and combine with bam file to do the final prediction.

violet-everygarden commented 6 days ago

Thank you for your prompt reply and help. I will try your suggestion.