ONT direct RNA with Tombo and MINES

YeoLab / MINES

(m)6A (I)dentification Using (N)anopor(E) (S)equencing

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ONT direct RNA with Tombo and MINES #4

Open akk01 opened 4 years ago

akk01 commented 4 years ago

while processing the data with Tombo before using MINES, which reference fasta should be used for ONT direct RNA sequencing data - genome or cDNA fasta.

yefei521 commented 3 years ago

In Tombo [https://nanoporetech.github.io/tombo/modified_base_detection.html], It said that you should provide transcripts as reference when processing Nanopore DRS data (call m6A). About how to extract transcripts, TBtools may be help a lot for you. (exon + downstream + upstream)