There was an error that I couldn't solve when I was using the barcodecollapsepe.py script.
could you please do me a favor?
Traceback (most recent call last):
File "./barcodecollapsepe.py", line 134, in
main()
File "./barcodecollapsepe.py", line 127, in main
total_count, removed_count = barcode_collapse(options.bam, options.out_file)
File "./barcodecollapsepe.py", line 62, in barcode_collapse
samfile_read1 = itertools.islice(samfile1, 0, None, 2)
File "csamtools.pyx", line 1219, in csamtools.Samfile.iter (pysam/csamtools.c:12851)
NotImplementedError: can not iterate over samfile without header
There was an error that I couldn't solve when I was using the barcodecollapsepe.py script. could you please do me a favor?
Traceback (most recent call last): File "./barcodecollapsepe.py", line 134, in
main()
File "./barcodecollapsepe.py", line 127, in main
total_count, removed_count = barcode_collapse(options.bam, options.out_file)
File "./barcodecollapsepe.py", line 62, in barcode_collapse
samfile_read1 = itertools.islice(samfile1, 0, None, 2)
File "csamtools.pyx", line 1219, in csamtools.Samfile.iter (pysam/csamtools.c:12851)
NotImplementedError: can not iterate over samfile without header