Closed olgabot closed 9 years ago
Here's the gist: https://gist.github.com/olgabot/b3589fa517407053461f
Here's just the error text:
ERROR 11:35:06,676 FunctionEdge - Error: cutadapt -f fastq --match-read-wildcards --times 2 '-e' '0' '-O' '5' '--quality-cutoff' '6' '-m' '18' '-b' 'TCGTATGCCGTCTTCTGCTTG' '-b' 'ATCTCGTATGCCGTCTTCTGCTTG' '-b' 'CGACAGGTTCAGAGTTCTACAGTCCGACGATC' '-b' 'GATCGGAAGAGCACACGTCTGAACTCCAGTCAC' '-b' 'AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA' '-b' 'TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT' '-o' '/home/obotvinnik/projects/singlecell_pnms/analysis/singlecell_pnms_se_v1/CVN_01.polyATrim.adapterTrim.fastq' '/home/obotvinnik/projects/singlecell_pnms/analysis/singlecell_pnms_se_v1/CVN_01' > CVN_01.polyATrim.adapterTrim.metrics ERROR 11:35:06,790 FunctionEdge - Contents of /home/obotvinnik/projects/singlecell_pnms/analysis/singlecell_pnms_se_v1/CVN_01.polyATrim.adapterTrim.fastq.out: discarding /projects/ps-yeolab/software/anaconda-2.1.0_2015-01-20/bin from PATH prepending /projects/ps-yeolab/software/anaconda-2.1.0_2015-01-20/envs/olga/bin to PATH discarding /projects/ps-yeolab/software/anaconda-2.1.0_2015-01-20/bin from PATH prepending /projects/ps-yeolab/software/anaconda-2.1.0_2015-01-20/envs/olga/bin to PATH Traceback (most recent call last): File "/projects/ps-yeolab/software/bin/cutadapt", line 10, in <module> cutadapt.main() File "/home/yeo-lab/software/lib/python2.7/site-packages/cutadapt/scripts/cutadapt.py", line 769, in main reader = read_sequences(input_filename, quality_filename, colorspace=options.colorspace, fileformat=options.format) File "/home/yeo-lab/software/lib/python2.7/site-packages/cutadapt/scripts/cutadapt.py", line 247, in read_sequences return seqio.SequenceReader(seqfilename, colorspace, fileformat) File "/home/yeo-lab/software/lib/python2.7/site-packages/cutadapt/seqio.py", line 161, in SequenceReader return fastq_reader(file) File "/home/yeo-lab/software/lib/python2.7/site-packages/cutadapt/seqio.py", line 295, in __init__ file = xopen(file, "r") File "/home/yeo-lab/software/lib/python2.7/site-packages/cutadapt/xopen.py", line 47, in xopen return open(filename, mode) IOError: [Errno 2] No such file or directory: '/home/obotvinnik/projects/singlecell_pnms/analysis/singlecell_pnms_se_v1/CVN_01' Nodes: tscc-1-33 discarding /projects/ps-yeolab/software/anaconda-2.1.0_2015-01-20/bin from PATH prepending /projects/ps-yeolab/software/anaconda-2.1.0_2015-01-20/envs/olga/bin to PATH ERROR 11:35:06,808 QGraph - Uncaught error running jobs. java.lang.NoClassDefFoundError: org/broadinstitute/sting/queue/engine/QGraph$$anonfun$runJobs$3$$anonfun$apply$4 at org.broadinstitute.sting.queue.engine.QGraph$$anonfun$runJobs$3.apply(QGraph.scala:479) at org.broadinstitute.sting.queue.engine.QGraph$$anonfun$runJobs$3.apply(QGraph.scala:472) at scala.collection.immutable.HashSet$HashSet1.foreach(HashSet.scala:130) at scala.collection.immutable.HashSet$HashTrieSet.foreach(HashSet.scala:275) at org.broadinstitute.sting.queue.engine.QGraph.runJobs(QGraph.scala:472) at org.broadinstitute.sting.queue.engine.QGraph.run(QGraph.scala:156) at org.broadinstitute.sting.queue.QCommandLine.execute(QCommandLine.scala:171) at org.broadinstitute.sting.commandline.CommandLineProgram.start(CommandLineProgram.java:245) at org.broadinstitute.sting.commandline.CommandLineProgram.start(CommandLineProgram.java:152) at org.broadinstitute.sting.queue.QCommandLine$.main(QCommandLine.scala:62) at org.broadinstitute.sting.queue.QCommandLine.main(QCommandLine.scala)
Have you run into this before? Does this mean you need to create a folder for each sample?
wait ack my sample manifest file is formatted incorrectly.. I thought I fixed that
Here's the gist: https://gist.github.com/olgabot/b3589fa517407053461f
Here's just the error text:
Have you run into this before? Does this mean you need to create a folder for each sample?