In the result, there are mainly .idr.out, .idr.out.normed.bed and .custombed files. According to Supplementary Protocol 2: eCLIP-seq Processing Pipeline, the .custombed file should be the final result and contain individual replicate information. However, in the Readme, file *.custombed is told that it cannot be used. So in which file should I use the results to annotate genes? If I can get a reply, I would be extremely grateful.
The custombed files should have the same information as what's in the paper, so they can be used. However since BED is a more acceptable standard, we recommend using that as a "final" result.
In the result, there are mainly .idr.out, .idr.out.normed.bed and .custombed files. According to Supplementary Protocol 2: eCLIP-seq Processing Pipeline, the .custombed file should be the final result and contain individual replicate information. However, in the Readme, file *.custombed is told that it cannot be used. So in which file should I use the results to annotate genes? If I can get a reply, I would be extremely grateful.