differential 3'UTR - Githubissues

YiPeng-Gao / scDaPars

Dynamic Analysis of Alternative Polyadenylation from single-cell RNA-seq (scDaPars)

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differential 3'UTR #12

Closed BenxiaHu closed 3 years ago

BenxiaHu commented 3 years ago

Hello, I just ran scDaPars and got the results, it seems that scDaPars does not perform differential 3'UTR between differential cells or clusters.

YiPeng-Gao commented 3 years ago

Hello, I just ran scDaPars and got the results, it seems that scDaPars does not perform differential 3'UTR between differential cells or clusters.

scDaPars output PDUI values. If you want to figure out differential 3'UTR, you can check the updated manuscript in https://genome.cshlp.org/content/early/2021/05/25/gr.271346.120.abstract for how we identify differential 3'UTR.

BenxiaHu commented 3 years ago

thanks. so here you just compare the entire PDUI between different cell types, not PDUI of the same genes between different cell types, right?

YiPeng-Gao commented 3 years ago

thanks. so here you just compare the entire PDUI between different cell types, not PDUI of the same genes between different cell types, right?

Actually, We only compare PDUIs for the same genes between different cell types. Here PDUI celltype1 means average PDUI for the gene of interest in cell type 1.