Use 50G bam file to run DaPars software, the result file contains more than 10,000 genes.
Split the 50G bam file into single cell bam files according to the barcode, and run DaPars software. The DaPars result file contains hundreds of genes.
Excuse me, after the bam file is split into a single cell bam file according to the barcode, do I need to modify some parameters? Can you give advice on reducing the number of genes so much?
Use 50G bam file to run DaPars software, the result file contains more than 10,000 genes. Split the 50G bam file into single cell bam files according to the barcode, and run DaPars software. The DaPars result file contains hundreds of genes. Excuse me, after the bam file is split into a single cell bam file according to the barcode, do I need to modify some parameters? Can you give advice on reducing the number of genes so much?