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YiPeng-Gao / scDaPars

Dynamic Analysis of Alternative Polyadenylation from single-cell RNA-seq (scDaPars)
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Dapars_hESC_combined_all_chromosome.txt #4

Closed ghost closed 3 years ago

ghost commented 3 years ago

Run DaPars2, Output format, /scDaPars/Example/Dapars_hESC_combined_all_chromosome.txt comes out with a column of numbers. What should I do with the data after Dapars2 runs? Dapars_hESC_combined_all_chromosome.txt What information is the number in the first column? image

ghost commented 3 years ago

Use https://github.com/3UTR/DaPars2 test data to generate ./DaPars2_Test_data_chr3/DaPars2_Test_data_result_temp.chr3.txt, use ./DaPars2_Test_data_chr3/DaPars2_Test_data_result_temp.chr3.txt to run the scDAPA software, an error appears as shown in the figure. After searching for the reason, I found that the https://github.com/YiPeng-Gao/scDaPars/issues/ sample file has more information in the first column than the result file of DaPars2 operation, so I want to consult how the information in the first column is generated. ./DaPars2_Test_data_chr3/DaPars2_Test_data_result_temp.chr3.txt 19K image image

ghost commented 3 years ago

There is no mention of a column of information in the literature， image

YiPeng-Gao commented 3 years ago

Use https://github.com/3UTR/DaPars2 test data to generate ./DaPars2_Test_data_chr3/DaPars2_Test_data_result_temp.chr3.txt, use ./DaPars2_Test_data_chr3/DaPars2_Test_data_result_temp.chr3.txt to run the scDAPA software, an error appears as shown in the figure. After searching for the reason, I found that the https://github.com/YiPeng-Gao/scDaPars/issues/ sample file has more information in the first column than the result file of DaPars2 operation, so I want to consult how the information in the first column is generated. ./DaPars2_Test_data_chr3/DaPars2_Test_data_result_temp.chr3.txt 19K image image

I think this is because the example file only have 2 samples.... it does not make sense to cluster 2 samples..

YiPeng-Gao commented 3 years ago

The example file has been updated.