Closed ghost closed 3 years ago
Is the flow chart missing 3 steps and not displayed?
- selected reads with correct unique molecular identifier (UMI) using Drop-seq tools FilterBAM (Macosko et al. 2015)
- remove reads with duplicated UMIs using UMI-tools dedup (Smith et al. 2017).
- merged reads originated from same cells together and generated one BAM file for each single cell
This is standard procedure for any 10X (UMI-based protocol) single cell procedure, not specific to scDaPars that is why it is not included in the pipeline.
Is the flow chart missing 3 steps and not displayed?