Closed gokulan-15 closed 3 years ago
In detail, please look at the original QTL-seq papaer by Takagi et al. (2013).
'--parent' option in QTL-seq is to input a sequence file for either of the parents used to create bulked populations. '--cultivar' option in MutMap is to input a sequence file for NOT mutagenized parent to create bulked populations.
These options are used to polarize the bulked alleles.
Thanks for the clarification, @YuSugihara!
Hi, @YuSugihara!
I would like to be clarified on which sequence to input for the
--parent
option in QTL-seq - Is it the parent that shows the desired phenotype or the one that does not?Similarly, what should be the input for
--cultivar
in MutMap: wildtype or the mutant sequence? Your reply to these would be of great help.Best! Gokulan.