YuSugihara / QTL-seq

QTL-seq pipeline to identify causative mutations responsible for a phenotype
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qtlseq qtl-seq error occur #45

Open HaNjIhEoN1 opened 11 months ago

HaNjIhEoN1 commented 11 months ago

hello I have a question about some error

I downloaded the test file and ran it in the way of example 2.

qtlseq -r reference.fasta -p parent.1.fastq,parent.2.fastq -b1 bulk_1.1.fastq,bulk_1.2.fastq -b2 bulk_2.1.fastq,bulk_2.2.fastq -n1 20 -n2 20 -o example_dir -T

[QTL-seq:2023-09-18 15:04:12] start to run QTL-seq. [QTL-seq:2023-09-18 15:04:12] maximum number of threads which you can use is up to 8. Traceback (most recent call last): File "/home/jjw/miniconda3/envs/qtl/bin/qtlseq", line 33, in sys.exit(load_entry_point('qtlseq', 'console_scripts', 'qtlseq')()) File "/home/jjw/QTL-seq/qtlseq/qtlseq.py", line 173, in main QTLseq(args).run() File "/home/jjw/QTL-seq/qtlseq/qtlseq.py", line 36, in init self.link_ref() File "/home/jjw/QTL-seq/qtlseq/qtlseq.py", line 63, in link_ref os.symlink(path_to_ref, sym_ref) OSError: [Errno 38] Function not implemented: '/media/jjw/seagate/hy/fasta/qtlseq_ref.fasta' -> 'test/10_ref/qtlseq_ref.fasta'

However, an error occurred. The error contents are as follows.

OSError: [Errno 38] Function not implemented: 'qtlseq_ref.fasta' -> 'example/10_ref/qtlseq_ref.fasta'

Please tell me the solution or the cause of the problem