YuSugihara / QTL-seq

QTL-seq pipeline to identify causative mutations responsible for a phenotype
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QTLplots have missing chromosomes #46

Open StephenSiazon opened 11 months ago

StephenSiazon commented 11 months ago

Hello! I am having problems with generating my QTLplot.   I have run QTLseq for my sequences and the initial QTLplot from this run has does not have a smooth red line. So I decided to run QTLplot using the vcf file from my QTLseq run using the code below:   qtlplot -v qtlseq.vcf.gz \         -o bsa_plot \         -n1 10 \         -n2 10 \         -w 2000 \         -s 100

Here’s the picture: delta_SNPindex

I tried changing the -w to 1000 and the chromosomes are okay. But running the same -w 1000 for the second time, chromosomes 1-5 are lost (picture below). Same with when I run -w 3000, first one is okay and the rerun has missing chromosomes.

-w 1000 rerun delta_SNPindex

-w 3000 rerun delta_SNPindex

I don't understand why. Hope you could help. Thank you very much! :)