Group with replicates, in other words, singleton samples, cause Qiime2 diversity analysis to fail. Moreover, sample filtering based on numbers of reads with taxonomy could result in singleton samples that are not intended, which in turn, cause a problem in diversity analysis.
A better metadata filtering code, and an additional sample filtering step to ensure that there are no singleton samples fed into the diversity analysis, are needed.
Group with replicates, in other words, singleton samples, cause Qiime2 diversity analysis to fail. Moreover, sample filtering based on numbers of reads with taxonomy could result in singleton samples that are not intended, which in turn, cause a problem in diversity analysis. A better metadata filtering code, and an additional sample filtering step to ensure that there are no singleton samples fed into the diversity analysis, are needed.