Zymo-Research / figaro

An efficient and objective tool for optimizing microbiome rRNA gene trimming parameters
GNU General Public License v3.0
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ValueError occuring when inputing v3-v4 .fastq files #53

Open elishafreedman opened 1 year ago

elishafreedman commented 1 year ago

Hi,

I have been experiencing the following error, my code is as follows:

python3 figaro.py -i /path/to/Directory -o figaro_report -a 300 -f 1 -r 1 -F illumina

I am dealing with Illumina MiSeq data which has already had the primers removed. my forward and reverse sequences are all 300 bp long I cropped them in trimmomatic beforehand so they would be the same length of 300bp.

This is the error I receive: raise self._value ValueError: setting an array element with a sequence. The requested array has an inhomogeneous shape after 1 dimensions. The detected shape was (19251,) + inhomogeneous part.

Thanks in advance! any help would be greatly appreciated!