pycoQC computes metrics and generates Interactive QC plots from the sequencing summary report generated by Oxford Nanopore technologies basecaller (Albacore/Guppy)
Currently, the code looks at the enumerate index i to see if a fast5 file was found in the given directory. However, if a directory does not have any files in it, then the enumeration loop won't get started and the i variable will actually be unbound, so the code as written would raise an UnboundLocalError. If there is a single fast5 file in the directory, then i will be valid, but it will be ==0, causing the current code to error saying that no fast5 files were found.
I've updated the logic to use a simple flag to indicate when we have actually inserted a fast5 file into the Queue during this loop.
Currently, the code looks at the enumerate index
ito see if a fast5 file was found in the given directory. However, if a directory does not have any files in it, then the enumeration loop won't get started and theivariable will actually be unbound, so the code as written would raise anUnboundLocalError. If there is a single fast5 file in the directory, theniwill be valid, but it will be==0, causing the current code to error saying that no fast5 files were found.I've updated the logic to use a simple flag to indicate when we have actually inserted a fast5 file into the Queue during this loop.