a-slide / pycoQC

pycoQC computes metrics and generates Interactive QC plots from the sequencing summary report generated by Oxford Nanopore technologies basecaller (Albacore/Guppy)
https://a-slide.github.io/pycoQC/
GNU General Public License v3.0
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Discrepancies between ToulligQC and PycoQC #151

Closed sklages closed 4 months ago

sklages commented 4 months ago

There are quite significant discrepancies between pycoQC and ToulligQC numbers and plots (based on pass data) when run on the same dataset.

That is probably due to the definition of "pass" for a read, that will most likely influence (all) subsequent calculations based on "pass" reads.

Can you provide a definition of what it needs for a read to be considered "pass" by pycoQC?