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aakechin / NGS-PrimerPlex

NGS-PrimerPlex is a high-throughput tool for mupltiplex primer design
GNU General Public License v3.0
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-ref and -wgref arguments are required #39

Open luzhouyangge opened 2 years ago

luzhouyangge commented 2 years ago

Hi, I am using the non-docker version, installed with v1.3.3.

When run the text.py, error occurs:

1. Testing extraction of genome regions...

ERROR (1)! If the docker image without reference genome or non-docker version is used, -ref and -wgref arguments are required.

How to solve the error?

Thank you

aakechin commented 2 years ago

@luzhouyangge you need to use both parameters -ref and -wgref. Without them, the program doesn't know which reference genome you are going to use.

luzhouyangge commented 2 years ago
屏幕快照 2022-10-25 下午5 49 56

Please list the files in hg19. Thank you!

luzhouyangge commented 2 years ago
屏幕快照 2022-10-25 下午5 53 22

Always error

aakechin commented 2 years ago

@luzhouyangge you need to download and prepare GenBank files to use getGeneRegions.py script as it is described at https://github.com/aakechin/NGS-PrimerPlex#reference-genome Also, it is described in details at Wiki pages: https://github.com/aakechin/NGS-PrimerPlex/wiki/NGS-PrimerPlex-installation-in-linux-as-a-standalone-tool For example, in my hg19 directory there are the following GenBank files: chr10.gb chr11.gb chr12.gb chr13.gb chr14.gb chr15.gb chr16.gb chr17.gb chr18.gb chr19.gb chr1.gb chr20.gb chr21.gb chr22.gb chr2.gb chr3.gb chr4.gb chr5.gb chr6.gb chr7.gb chr8.gb chr9.gb chrM.gb chrX.gb chrY.gb