There is a KeyError in the running result:
Creating primer3 parameters for external primers design, combination variant 1...
Constructing external primers...
11.11%Traceback (most recent call last):
File "NGS_primerplex.py", line 3707, in
leftGC,rightGC,outputInternalPrimers[curRegionName][7]-(start+primersCoords[0][1])+1,
KeyError: 'EGFRG719S'
The -regions is : chr7 55259515 55259515 L858R 1 B NotW chr7 55241707 55241707 G719S 1 B NotW chr7 55249071 55249071 T790M 1 B NotW
run the following commands: python3 NGS_primerplex.py \ -regions Primers/cs_glf_r.csv \ -ref hg19/ucsc.hg19.fasta \ -embedded \ -minampllen 90 \ -optampllen 120 \ -maxampllen 130 \ -blast \ -snps \ -nucs 10 \ -dbsnp hg19/common_all_20180423_hg19.vcf.gz \ -primernum1 10 \ -minprimermelt 59 \ -maxprimermelt 61 \ -optprimermelt 60
There is a KeyError in the running result: Creating primer3 parameters for external primers design, combination variant 1... Constructing external primers... 11.11%Traceback (most recent call last): File "NGS_primerplex.py", line 3707, in leftGC,rightGC,outputInternalPrimers[curRegionName][7]-(start+primersCoords[0][1])+1, KeyError: 'EGFRG719S'
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