Error in detecting specifity!

[HFyange]

Running BWA... Processing SAM-file... 100.0%

Total number of nonspecific regions: 1779212

Searching for nonspecific amplicons that are formed by designed primer pairs...

Number of specific primer pairs: 15996

Removing unspecific primer pairs... Searching for nonspecific amplicons that are formed by primers from different primer pairs... 94.13%Segmentation fault Process ForkPoolWorker-12: Traceback (most recent call last): File "/usr/lib/python3.5/multiprocessing/pool.py", line 125, in worker put((job, i, result)) File "/usr/lib/python3.5/multiprocessing/queues.py", line 355, in put self._writer.send_bytes(obj) File "/usr/lib/python3.5/multiprocessing/connection.py", line 200, in send_bytes self._send_bytes(m[offset:offset + size]) File "/usr/lib/python3.5/multiprocessing/connection.py", line 404, in _send_bytes self._send(header + buf) File "/usr/lib/python3.5/multiprocessing/connection.py", line 368, in _send n = write(self._handle, buf) BrokenPipeError: [Errno 32] Broken pipe

During handling of the above exception, another exception occurred:

Traceback (most recent call last): File "/usr/lib/python3.5/multiprocessing/process.py", line 249, in _bootstrap self.run() File "/usr/lib/python3.5/multiprocessing/process.py", line 93, in run self._target(*self._args, **self._kwargs) File "/usr/lib/python3.5/multiprocessing/pool.py", line 130, in worker put((job, i, (False, wrapped))) File "/usr/lib/python3.5/multiprocessing/queues.py", line 355, in put self._writer.send_bytes(obj) File "/usr/lib/python3.5/multiprocessing/connection.py", line 200, in send_bytes self._send_bytes(m[offset:offset + size]) File "/usr/lib/python3.5/multiprocessing/connection.py", line 404, in _send_bytes self._send(header + buf) File "/usr/lib/python3.5/multiprocessing/connection.py", line 368, in _send n = write(self._handle, buf) BrokenPipeError: [Errno 32] Broken pipe