aammd
commented
6 years ago Just wanted to triple check that I've addressed everything. Here are comments by DS
- [x] P1 line 14. Insert Louca et al. 2016 in ref
- [x] P2 line 38. References after word “further”
- [x] P4 line 74. After here, suggest move para that is currently p5 line 88-95.
- [x] Figure 1. X axis: “Gradient of increasing organism size”; add legend explaining each of three pictures, e.g. “[damselfly image] macroinvertebrates xx-yy um”; line 105 add “spatially” before “biased. In genera, the figures need to now go to end of ms, and the figure legends need to be all on one page. See Ecology instructions to authors.
- [x] P6 line 110. Change “expt design” to “Study site”. This will be beginning of methods section.
- [x] P6 line 118. Insert header after: “Experimental design
- [x] P7 line 134 insert “based on previous studies (Farjalla et al. 2012)”
- [x] P8 line 140 what size filter paper?
- [x] P8 ;ine 142. Insert after “(OTUs)”. Although this yieds fairly coarse-level separation of taxonomic groups, we used this method to be able to replicate the original results of Farjalla et al. (2012). We demonstrate later that our results are qualitatively similar to that obtained by marker gene sequencing (Louca et al. 2016).
- [x] P8 line 151. Change Bromeliad preparation to “Community assembly in bromeliads”
- [x] P10 line 194 before community composition insert “the unmanipualated”
- [x] P10 line 197 replce “at the beginning” by “befre the homogenization”
- [x] Table 1, capitalize row names
- [x] Figures – lets add up cost of colour, looks like nothing totally needs to be in colour...
- [x] Figure 3. Change initial and final to “before homogenization” and “12 days after homogenization”
- [x] P15 line 270. Because the positive association between organism size with dispersal limitation masked the signal of organism size on environmental filtering
- [x] P15 line 271 results of such regional studies
- [x] P16 line 287 replace Chapter 2 with reference to your thesis
- [x] P16 line 288 effects not effectd
- [x] P17 line 298. After “the environment” : “In fact, although bromeliad microbes do demonstrate non-neutral and non-random species associations, these patterns appear to occur independent of the environment (Farjalla et al. 2012; Louca et al. 2016).
- [x] P 18 line 323-327, starting at word which: “which used the higher-resolution method of marker gene sequencing to identify microbial taxa, also found that environmental differences between bromeliads explained little variation in taxonomic composition of their microbial communities (Louca et al. 2016). Importantly, the percent of variation explained by the environment in this metagenomics analysis was similar across a range of taxonomic resolutions, from sub-genera to class, suggesting that we did not overlook environmental filtering by using the coarser taxonomic resolution of DGGE methods. In fact, both the metagenomic analysis of Louca et al. (2016) and the DGGE analysis of Farjalla et al. (2012) showed the same pattern of non-random species associations in bromeliad microbes despite little environmental filtering”.
- [x] P20 line 358 replace ours with bromeliads
L64 -- reference for bacteria using env DNA L60 -- ref for smaller animals have spores(?) L122 L296 -- to Methods? Is it not mentioned there? L337 - why the "Fig3" in the text?? it .. isn't Resolution on figure is a little low? Drop the subtitles? I kind of like them..