search

aarsonline / aarsonline.github.io

AARS Online - an interactive database of aminoacyl-tRNA synthetase sequences, structures, and taxonomy
GNU General Public License v3.0
1 stars 0 forks source link

Where does the R. palustris ProRS belong? #53

Open jordandouglas opened 10 months ago

jordandouglas commented 10 months ago

This protein may potentially fall under ProRS-M, but it is unclear. It has a short insertion in place of the editing domain

https://www.rcsb.org/structure/2i4o

Thanks Oscar ;)

jordandouglas commented 10 months ago

The three ProRS families are summarised this figure: https://www.sciencedirect.com/science/article/pii/S0969212606003558?via%3Dihub#fig1

"Structures of Two Bacterial Prolyl-tRNA Synthetases with and without a cis-Editing Domain"

jordandouglas commented 10 months ago

My phylogenetic analysis places PDB 2I4O in the ProRS-B clade, not ProRS-M. This means the editing domain must have been lost at least 2x. Once in bacteria and once in organelles.

image

jordandouglas commented 10 months ago

As far as I am aware, the ProRS-M clade does not have any solved structures