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ablab / rnaquast

Quality assessment of de novo transcriptome assemblies from RNA-Seq data
http://cab.spbu.ru/software/rnaquast
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"Unnanotated" and "Predicted genes" outputs doubts #6

Closed MartaBenegas closed 3 years ago

MartaBenegas commented 3 years ago

Hi rnaQUAST team! First of all, thank you for your work. I really like the tool so far! I have a few questions regarding the output tough:

  1. I've run the analysis without the --busco and --gene_mark options and yet I have a "Predicted genes" stat in my output. How is this number calculated? I haven't been able to find it in the manual.
  2. The "Unnanotated" stat is defined in the manual as "total number of transcripts that do not cover any isoform from the database". However, I have float numbers as a result, suggesting that it is more a fraction or a ratio. How is this number calculated?

Here you have my short report: short_report.pdf

Regards, Marta.

andrewprzh commented 3 years ago

Hi, sorry for the delay - something was wrong with GitHub notifications.

I seems like we've seen this bug previously. Which version of rnaQUAST do you use? Could you send the log file?

Best Andrey

andrewprzh commented 3 years ago

I seems like this bug does not reproduce in the latest version rnaQUAST 2.2.0.