Closed sahilrishav2 closed 2 months ago
The log reads:
0:41:14.159 7030M / 70G ERROR General (single_read.hpp : 74) Invalid read: length of sequence should equal to length of quality line
So your input reads are corrupted (the long ones).
Description of bug
Hi,
I am trying to use SPAdes tool for assembling the fungi genome but an unexpected error is showing:
== Error == system call for: "['/data/jammu-univ/software/SPAdes-3.15.5-Linux/bin/spades-core', '/data/jammu-univ/data/ayushi_data/JNU/Endo_CSE15_sample/Pacbio_data/hybrid_spades_assembly/output_folder/K77/configs/config.info']" finished abnormally, OS return value: 34
Kindly help me regarding this concern.
Thank You
spades.log
spades.log
params.txt
bin/spades.py -1 /data/jammu-univ/data/ayushi_data/JNU/Endo_CSE15_sample/illumina_data/Endo1_S101_R1_001.fastq -2 /data/jammu-univ/data/ayushi_data/JNU/Endo_CSE15_sample/illumina_data/Endo1_S101_R2_001.fastq --pacbio /data/jammu-univ/data/ayushi_data/JNU/Endo_CSE15_sample/Pacbio_data/Endo_combine.fastq -o /data/jammu-univ/data/ayushi_data/JNU/Endo_CSE15_sample/Pacbio_data/hybrid_spades_assembly/output_folder -t 80 -m 500
SPAdes version
SPAdes v3.15.5
Operating System
linux
Python Version
Python 3.11.8
Method of SPAdes installation
binaries
No errors reported in spades.log