ablab / spades

SPAdes Genome Assembler
http://ablab.github.io/spades/
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sequence name is empty #1349

Closed mmani4 closed 2 months ago

mmani4 commented 2 months ago

Description of bug

I run spades for the assembly and tried quast. I am getting this error

ERROR! Skipping assembly1/spades-original/corrected/B54_R100.0_0.cor.fastq.gz because >sequence_name field is empty for the entry starting with "@K00124:531:HC23JBBX". can you assist me to resolve this isssue

spades.log

spades (1).log

params.txt

params.txt

SPAdes version

SPAdes version: 4.0.0

Operating System

Linux-4.18.0-477.21.1.el8_8.x86_64-x86_64-with-glibc2.28

Python Version

3.12.4

Method of SPAdes installation

conda

No errors reported in spades.log

asl commented 2 months ago

There are no errors in the attached spades.log file. Will you please attach the proper log with error?

mmani4 commented 2 months ago

Hi, I finished running spades, but when I tried to run quast, it shows like there is an error in the assembly file (I mean the spades output)

The error is ERROR! Skipping assembly1/spades-original/corrected/B54_R_unpaired00.0_0.cor.fastq.gz because >sequence_name field is empty for the entry starting with @.***:531:HC23JBBX".

Best, Monika

From: Anton Korobeynikov @.> Sent: Wednesday, August 7, 2024 12:05 PM To: ablab/spades @.> Cc: Monika Mani @.>; Author @.> Subject: Re: [ablab/spades] sequence name is empty (Issue #1349)

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There are no errors in the attached spades.log file. Will you please attach the proper log with error?

- Reply to this email directly, view it on GitHubhttps://github.com/ablab/spades/issues/1349#issuecomment-2273819153, or unsubscribehttps://github.com/notifications/unsubscribe-auth/A3NADUV6XF2TZAMR7APA3QTZQJAS5AVCNFSM6AAAAABMEW2RXSVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZDENZTHAYTSMJVGM. You are receiving this because you authored the thread.Message ID: @.***>

asl commented 2 months ago

The error is ERROR! Skipping assembly1/spades-original/corrected/B54_R_unpaired00.0_0.cor.fastq.gz because >sequence_name field is empty for the entry starting with @.***:531:HC23JBBX".

It seems you are running QUAST on reads, not assembler output.