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ablab / spades

SPAdes Genome Assembler
http://ablab.github.io/spades/
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Verification of expression 'cov_.size() > 10' failed in function 'void coverage_model::KMerCoverageModel::Fit()' #1361

Closed caiji-lh closed 2 months ago

caiji-lh commented 2 months ago

Description of bug

I am analyzing metagenomic data from the CAMI challenge, but I encountered some errors when running SPAdes. I've reviewed similar issues, but I’m still a bit confused and would appreciate some assistance.

spades.log

spades.log

params.txt

params.txt

SPAdes version

SPAdes v3.11.1

Operating System

Linux-5.15.0-112-generic-x86_64-with-Ubuntu-18.04-bionic

Python Version

No response

Method of SPAdes installation

manual

No errors reported in spades.log

asl commented 2 months ago

The log reads:

Invalid kmer coverage histogram, make sure that the coverage is indeed uniform'.

So you are assembling a dataset with non-uniform coverage using multicell mode that expects uniform coverage, this is not going to work.

Also, you are using terrible ancient SPAdes version