Hi everyone,
I encountered an error while using SPAdes-3.15.5 to assemble a diploid genome (~560 Mb) with 0.67% heterozygosity. About 124 GB of corrected reads were provideed to the assembler.
Is there anything that can be adjusted to achieve a complete assembly?
Description of bug
Hi everyone, I encountered an error while using SPAdes-3.15.5 to assemble a diploid genome (~560 Mb) with 0.67% heterozygosity. About 124 GB of corrected reads were provideed to the assembler. Is there anything that can be adjusted to achieve a complete assembly?
spades.log
spades.log
params.txt
~/biosoft/SPAdes-3.15.5-Linux/bin/spades.py --cov-cutoff 5 --isolate -t 32 -m 1200 -1 N199_spades/corrected/N199_1.clean.fq.00.0_0.cor.fastq.gz -2 N199_spades/corrected/N199_2.clean.fq.00.0_0.cor.fastq.gz -o N199_spades_5
SPAdes version
SPAdes-3.15.5
Operating System
CentOS Linux 7 (Core)
Python Version
Python 3.10.8
Method of SPAdes installation
binaries
No errors reported in spades.log