Closed sukesh14 closed 3 years ago
Hi, most likely your ROOTSYS variable is set incorrectly.
Mayo Clinic, Harwick 3-12 200 1st street SW, Rochester, MN 55905 tel: +1-(507)-538-0978 fax: +1-(507)-284-0745
Dear Sir,
Thanks for your prompt reply. The path for root system and library is mentioned as below before installing the CNVnator v0.27 or v 0.3. I don't know whether i am mentioning the correct path or not.
export ROOTSYS=/home/sukesh/Downloads/root-6.08.04/ export LD_LIBRARY_PATH=$LD_LIBRARY_PATH:$ROOTSYS/lib.
After mentioning the above paths, i still get the errors.
/home/sukesh/Downloads/root-6.08.04/include/libcpp_string_view.h:776:53: error: invalid type in declaration before ‘;’ token
typedef basic_string_view
Thanks Sukesh
Hi, could be incompatibility with compiler. Are you able to run ROOT?
Mayo Clinic, Harwick 3-12 200 1st street SW, Rochester, MN 55905 tel: +1-(507)-538-0978 fax: +1-(507)-284-0745
Dear Sir.
I can run the ROOT from bin folder of ROOT using the command ./root. In the bashrc i mentioned path for thisroot.sh.
Thanks Sukesh
From: "abyzov" notifications@github.com To: "abyzovlab/CNVnator" CNVnator@noreply.github.com Cc: "sukesh14" sukesh.k@ktr.srmuniv.ac.in, "Author" author@noreply.github.com Sent: Wednesday, March 22, 2017 10:33:01 PM Subject: Re: [abyzovlab/CNVnator] Issue installing CNVnator (#59)
Hi, could be incompatibility with compiler. Are you able to run ROOT?
Alexej Abyzov, Ph.D. Senior Associate Consultant, Assistant Professor of Biomedical Informatics, Department of Health Sciences Research, Center for Individualized Medicine, Mayo Clinic
Mayo Clinic, Harwick 3-12 200 1st street SW, Rochester, MN 55905 tel: +1-(507)-538-0978 fax: +1-(507)-284-0745
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HI, which compiler are your using?
Mayo Clinic, Harwick 3-12 200 1st street SW, Rochester, MN 55905 tel: +1-(507)-538-0978 fax: +1-(507)-284-0745
Dear Sir,
Thanks for your reply. I am using g++ v4.8.4 and gcc 4.8.4 compilers.
Thanks Sukesh
Dear Sir,
I installed CNVnator using cnvnator packager. (https://github.com/indraniel/cnvnator-packager). Can i use this software for plants?
Great! Currently CNVnator is intended for diploid genomes. If the ploidy of plants you are aiming at studying is higher then CNVnator will have limited abilities.
Mayo Clinic, Harwick 3-12 200 1st street SW, Rochester, MN 55905 tel: +1-(507)-538-0978 fax: +1-(507)-284-0745
Dear Sir,
Thank you for your information. I tried to predict the cnvs for rice genome. According to tutorial, i ran the first command to extract the regions for each chromosome separately using bam files. Now when i run the first step using the below command i did not get any error and one root file is created.
First command
./cnvnator -root newseec8.root -genome chr08.fasta -chrom chr08 -tree seechr08.bam
new root file is generated, but i could not know how to visualize this file in root software.
But, for second step for histogram - when i run this using the below command
./cnvnator -root newseec8.root -genome chr08.fasta -chrom chr08 -his 100
It gives me below message
Allocating memory ... Done. Calculating histograms with bin size of 100 for 'chr08' ... Making GC histogram for 'chr08' ... Can't open file with chromosome sequence. No chromosome/contig information parsed. Sequence length (0) is different from expectation (28443022) for 'chr08'. Doing nothing! Done.
I just want to know what is the mistake i committed for generating the histogram. And another question i wanted to know how to set the bin size. I tried bin size 4 by reading your paper, but still i get the same above message. We did whole genome sequencing using 10x.
Thanks Sukesh
From: "abyzov" notifications@github.com To: "abyzovlab/CNVnator" CNVnator@noreply.github.com Cc: "sukesh14" sukesh.k@ktr.srmuniv.ac.in, "Author" author@noreply.github.com Sent: Tuesday, March 28, 2017 10:35:46 AM Subject: Re: [abyzovlab/CNVnator] Issue installing CNVnator (#59)
Great! Currently CNVnator is intended for diploid genomes. If the ploidy of plants you are aiming at studying is higher then CNVnator will have limited abilities.
Alexej Abyzov, Ph.D. Senior Associate Consultant, Assistant Professor of Biomedical Informatics, Department of Health Sciences Research, Center for Individualized Medicine, Mayo Clinic
Mayo Clinic, Harwick 3-12 200 1st street SW, Rochester, MN 55905 tel: +1-(507)-538-0978 fax: +1-(507)-284-0745
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Hi, files should be named as: chr1.fa, chr2.fa, etc.
Mayo Clinic, Harwick 3-12 200 1st street SW, Rochester, MN 55905 tel: +1-(507)-538-0978 fax: +1-(507)-284-0745
hello, sukesh14 @sukesh14 , I am running into exactly the same issue as you did, do you mind telling me how did you solve the issue at the end? is the compiler the reason causing problem ? Thanks a lot
Dear Sir,
No. I tried the normal way for installing cnvnator, it did not work out. Later, i found cnvnator packager and installed it (https://github.com/indraniel/cnvnator-packagerIt worked out).
Root version was also another problem so i changed the root version 6.08 which is compatible with cnvnator 0.32.
From: "dangpandora" notifications@github.com To: "abyzovlab/CNVnator" CNVnator@noreply.github.com Cc: "sukesh14" sukesh.k@ktr.srmuniv.ac.in, "Mention" mention@noreply.github.com Sent: Wednesday, May 10, 2017 1:33:41 AM Subject: Re: [abyzovlab/CNVnator] Issue installing CNVnator (#59)
hello, sukesh14 @sukesh14 , I am running into exactly the same issue as you did, do you minding telling me how did you solve the issue at the end? is the compiler the reason causing problem ? Thanks a lot
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@sukesh14 Thank you very much for the quick reply, it helps
Dear Sir,
I am predicting cnv regions for three rice genomes using three cnv softwares like pindel, cnvnator and breakdancer. We are considering the best results, if at least two softwares out of three predict the same results. I have a doubt, if we find overlap between CNVs reported by two softwares, should we take only overlapping regions for wet lab study or from smallest start coordinate to largest coordinate?
Thanks Sukesh
----- Original Message ----- From: "dangpandora" notifications@github.com To: "abyzovlab/CNVnator" CNVnator@noreply.github.com Cc: "sukesh14" sukesh.k@ktr.srmuniv.ac.in, "Mention" mention@noreply.github.com Sent: Wednesday, May 10, 2017 7:12:12 PM Subject: Re: [abyzovlab/CNVnator] Issue installing CNVnator (#59)
@sukesh14 Thank you very much for the quick reply, it helps
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Hi, breakdance and pindel have better breakpoint resolution. So, I would think they you should consider their breakpoints for downstream experiments.
Mayo Clinic, Harwick 3-12 200 1st street SW, Rochester, MN 55905 tel: +1-(507)-538-0978 fax: +1-(507)-284-0745
hello,i get this problem when i make.CNVnator
V0.3.2and root6.22
the erro:/app/root/include/ROOT/libcpp_string_view.h:275:63: error: 'numeric_limits' is not a member of 'std'
275 | size_type max_size() const _NOEXCEPT { return (_VSTD::numeric_limits~~~~~
/app/root/include/ROOT/libcpp_string_view.h:275:87: error: expected primary-expression before '>' token
275 | size_type max_size() const _NOEXCEPT { return (_VSTD::numeric_limits
Dear Sir,
Please find the below error while installing the CNVnator. Kindly help me to troubleshoot this error. I installed root v6 and CNVnator 0.27 and 0.3 versions on ubuntu 14.04
root@sukesh-Precision-Tower-7810:/home/sukesh/Downloads/CNVnator_v0.2.7/src# make g++ -pthread -m64 -DCNVNATOR_VERSION=\"v0.2.7\" -I/home/sukesh/Downloads/root-6.08.04/bin//include -Isamtools -c cnvnator.cpp -o obj/cnvnator.o In file included from cnvnator.cpp:8:0: HisMaker.hh:11:20: fatal error: TFrame.h: No such file or directory
include
compilation terminated. make: *** [obj/cnvnator.o] Error 1
Thanks Sukesh