tune the nucleus extraction (reseal) algorithm

acq4 / acq4

Data acquisition for patch clamp ephys / optogenetics / imaging experiments

http://www.acq4.org

MIT License

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tune the nucleus extraction (reseal) algorithm #230

Open outofculture opened 5 months ago

outofculture commented 5 months ago

once the resistance hits a good level (500MOhm?), we can ignore the rate of change

outofculture commented 2 months ago

~Try setting reseal->resealSuccessResistance to 500*MOhm and see if that behaves better.~

campagnola commented 2 months ago

At 500 MΩ, retract from tissue slowly and then we're done?

jessicatrnh commented 2 months ago

I like this feature (500 MOhm). So far the cell ends up at 1 Gohm anyway when quickly removed. need to consider reintroducing tear and stretch thresholds?

jessicatrnh commented 1 month ago

In nearly all cases where ReSeal performs "corrective" behavior by moving back towards the cell, the seal on the membrane is being lost...

outofculture commented 1 month ago

Does turning off the "move backwards" behavior (by setting the tear threshold to -10000) improve things?

jessicatrnh commented 1 month ago

I increase the tear threshold to -1000. Testing.