cellpose's 3d algorithm fails with our brightfield data. our XZ and YZ slices are riddled with diffraction that make cell identification extremely difficult
cellpose's 3d algorithm works well with our fluorescence data, but it's pretty slow (138s to process a 117x1024x1024 stack on an RTX 4070)
cellpose's 3d annotation is inadequate and tedious. it doesn't merge/copy between layers
cellpose's cyto3 model matches on unhealthy cells as much as healthy ones
building a 3d model without cellpose in the mix would require a great deal of training data