Find tail and plot specific read in multi FAST5 file

adnaniazi / tailfindr

An R package for estimating poly(A)-tail lengths in Oxford Nanopore RNA and DNA reads.

https://www.cbu.uib.no/valen/

GNU General Public License v3.0

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Find tail and plot specific read in multi FAST5 file #22

Closed manburst closed 3 years ago

manburst commented 3 years ago

Hello sir, Now I am working on nanopore project with multi FAST5. Due to my multi FAST5 file is contain 4000 reads in there and I interested some reads in there and want to plot the current of them. Is anyway to plot and find the tail of specific reads in multi fast5 file because I running on my pc and plot files will consume my space, also too long running time. Thanks in advance.

adnaniazi commented 3 years ago

You can split multifast5 into single fast5 files using ont-fast-api (https://github.com/nanoporetech/ont_fast5_api) from Oxford nanopore, and then give only those single fast5s for which you need the plots as input to tailfindr.