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adnaniazi / tailfindr

An R package for estimating poly(A)-tail lengths in Oxford Nanopore RNA and DNA reads.
https://www.cbu.uib.no/valen/
GNU General Public License v3.0
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Can you explain the normalization process? #37

Closed yeroslaviz closed 2 years ago

yeroslaviz commented 2 years ago

i was wondering how tailfindr calculate the average nucleotide translocation rate, which is the value used for the normalization.

I read the paper, but don't really understand what it means, samples per nucleotide. What does one regards here as sample? are these the single, unique reads mapped at each specific nucleotide position?

thanks

adnaniazi commented 2 years ago

You can find the information about samples_per_nt here: https://adnaniazi.com/website-assets/niazi_and_krause_2019_springer_book_chapter.pdf