Open anchormok opened 3 years ago
Hi, did you manage to solve this? Thanks!
Dear Developer, I have same problem. could you help me to solve it? genesKept <- geneFiltering(exprMat, scenicOptions=scenicOptions, minCountsPerGene=3.01ncol(exprMat), minSamples=ncol(exprMat)*.01) Maximum value in the expression matrix: 56505 Ratio of detected vs non-detected: 0.11 Number of counts (in the dataset units) per gene: Min. 1st Qu. Median Mean 3rd Qu. Max. 0 61 1276 42116 10761 101433110 Number of cells in which each gene is detected: Min. 1st Qu. Median Mean 3rd Qu. Max. 0 58 1108 6627 8445 68934
Number of genes left after applying the following filters (sequential): 11927 genes with counts per gene > 2089.35 11907 genes detected in more than 696.45 cells Using the column 'features' as feature index for the ranking database. 0 genes available in RcisTarget database
Hi, I have the same problem when using mouse single cell database, only 18 genes available in RcisTarget database. Can you have the methods to solve it? Thanks!
Did you solved it?
Dear developer, When I generated the geneFiltering function, I found that 0 genes available in RcisTarget database. The object "exprMat" is from Seurat object. And the above code is the same as your tutorial.
Maximum value in the expression matrix: 10 Ratio of detected vs non-detected: Inf Number of counts (in the dataset units) per gene: Min. 1st Qu. Median Mean 3rd Qu. Max. -1035.10 -121.23 -32.17 -12.17 70.13 1356.90 Number of cells in which each gene is detected: Min. 1st Qu. Median Mean 3rd Qu. Max. 147.0 655.0 714.0 682.2 763.0 1309.0
Number of genes left after applying the following filters (sequential): 11213 genes with counts per gene > 90 11213 genes detected in more than 30 cells 0 genes available in RcisTarget database
no genes available in RcisTarget database and I can't continue to run the SCENIC. Please help me find the problems. Thank you very much!