aertslab / SCENIC

SCENIC is an R package to infer Gene Regulatory Networks and cell types from single-cell RNA-seq data.
http://scenic.aertslab.org
GNU General Public License v3.0
394 stars 94 forks source link

No questions here, please! runSCENIC_3_scoreCells :Error in signif(trhAssignment, 3) #454

Open ManarHashemTaha opened 1 month ago

ManarHashemTaha commented 1 month ago

I got that error even if I change the ncores to ( 10 , 15,20,25 and 40) . I work on big matrix with 500Kcells and 20Kgenes

scenicOptions@settings$nCores <- 40 # Set the number of cores

3. Score GRN in the cells (with AUCell)

scenicOptions <- runSCENIC_3_scoreCells(scenicOptions, counts) 20:21 Step 3. Analyzing the network activity in each individual cell Number of regulons to evaluate on cells: 31 Biggest (non-extended) regulons: TFDP1 (105g) KLF4 (68g) XBP1 (46g) JUN (43g) EOMES (39g) MAFB (28g) ETS2 (21g) CEBPD (20g) FOSB (18g) FOS (13g) Quantiles for the number of genes detected by cell: (Non-detected genes are shuffled at the end of the ranking. Keep it in mind when choosing the threshold for calculating the AUC). min 1% 5% 10% 50% 100% 7 51 105 173 958 1578 Warning in .AUCell_calcAUC(geneSets = geneSets, rankings = rankings, nCores = nCores, : Using only the first 51 genes (aucMaxRank) to calculate the AUC. Using 31 cores with doMC. Error in signif(trhAssignment, 3) : non-numeric argument to mathematical function