prune2df report error

[zzzseeu]

Describe the bug When the program runs to the prune2df step, it will report an error: ArrowInvalid: Not a feather file. I read your previous discussion and thought it was a problem with the integrity of the database file, but I checked my database and there is no problem.

Steps to reproduce the behavior

1. Command run when the error occurred:

   
   import pandas as pd
   import numpy as np
   import os, glob
   import pickle

from arboreto.utils import load_tf_names from arboreto.algo import grnboost2

from pyscenic.rnkdb import FeatherRankingDatabase as RankingDatabase from pyscenic.utils import modules_from_adjacencies from pyscenic.prune import prune2df, df2regulons from pyscenic.aucell import aucell

import seaborn as sns

DATA_FOLDER="/home/xinzhou/scenic_test/" DATABASE_FOLDER = "/home/xinzhou/scenic_test/cisTarget_databases/"

DATABASES_GLOB = os.path.join(DATABASE_FOLDER, "mm9-*.mc9nr.genes_vs_motifs.rankings.feather")

MOTIF_ANNOTATIONS_FNAME = os.path.join(DATABASE_FOLDER, "motifs-v9-nr.mgi-m0.001-o0.0.tbl")

MM_TFS_FNAME = os.path.join(DATABASE_FOLDER, 'allTFs_mm.txt') SC_EXP_FNAME = os.path.join(DATA_FOLDER, "RNA-seq-counts_202207.csv")

ADJACENCIES_FNAME = os.path.join(DATA_FOLDER, "adjacencies.tsv") MODULES_FNAME = os.path.join(DATA_FOLDER, "modules.p") MOTIFS_FNAME = os.path.join(DATA_FOLDER, "motifs.csv") REGULONS_FNAME = os.path.join(DATA_FOLDER, "regulons.p")

N_SAMPLES = 12

ex_matrix = pd.read_csv(SC_EXP_FNAME, sep=',', header=0, index_col=0).T

tf_names = load_tf_names(MM_TFS_FNAME)

db_fnames = glob.glob(DATABASES_GLOB) def name(fname): return os.path.splitext(os.path.basename(fname))[0] dbs = [RankingDatabase(fname=fname, name=name(fname)) for fname in db_fnames]

adjancencies = grnboost2(expression_data=ex_matrix, tf_names=tf_names, verbose=True)

adjancencies.to_csv(ADJACENCIES_FNAME, index=False, sep='\t')

adjancencies = pd.read_csv('/home/xinzhou/scenic_test/adjacencies.tsv', sep='\t')

modules = list(modules_from_adjacencies(adjancencies, ex_matrix))

with open(MODULES_FNAME, 'wb') as f:

pickle.dump(modules, f)

with open(MODULES_FNAME, 'rb') as f: modules = pickle.load(f) df = prune2df(dbs, modules, MOTIF_ANNOTATIONS_FNAME)

2. Error encountered:
<!-- Please specify the **complete** error message (if applicable, otherwise delete this block): -->
```pytb
ArrowInvalid                              Traceback (most recent call last)
/tmp/ipykernel_2294546/3264675668.py in <module>
----> 1 df = prune2df(dbs, modules, MOTIF_ANNOTATIONS_FNAME)

/home/miniconda3/envs/scenic/lib/python3.7/site-packages/pyscenic/prune.py in prune2df(rnkdbs, modules, motif_annotations_fname, rank_threshold, auc_threshold, nes_threshold, motif_similarity_fdr, orthologuous_identity_threshold, weighted_recovery, client_or_address, num_workers, module_chunksize, filter_for_annotation)
    349     return _distributed_calc(rnkdbs, modules, motif_annotations_fname, transformation_func, aggregation_func,
    350                              motif_similarity_fdr, orthologuous_identity_threshold, client_or_address,
--> 351                              num_workers, module_chunksize)
    352 
    353 

/home/miniconda3/envs/scenic/lib/python3.7/site-packages/pyscenic/prune.py in _distributed_calc(rnkdbs, modules, motif_annotations_fname, transform_func, aggregate_func, motif_similarity_fdr, orthologuous_identity_threshold, client_or_address, num_workers, module_chunksize)
    298         if client_or_address == "dask_multiprocessing":
    299             # ... via multiprocessing.
--> 300             return create_graph().compute(scheduler='processes', num_workers=num_workers if num_workers else cpu_count())
    301         else:
    302             # ... via dask.distributed framework.

/home/miniconda3/envs/scenic/lib/python3.7/site-packages/dask/base.py in compute(self, **kwargs)
    288         dask.base.compute
    289         """
--> 290         (result,) = compute(self, traverse=False, **kwargs)
    291         return result
    292 
...
---> 84             raise ArrowInvalid(message)
     85         elif status.IsIOError():
     86             # Note: OSError constructor is

ArrowInvalid: Not a feather file

Please complete the following information:

- pySCENIC version: 0.11.1
- Installation method: pip
- Run environment: Jupyter notebook
- OS: Ubuntu
- Package versions: 
aiohttp==3.8.3
aiosignal==1.3.1
arboreto==0.1.6
async-timeout==4.0.2
asynctest==0.13.0
attrs==22.2.0
backcall==0.2.0
bokeh==2.4.3
boltons==21.0.0
Bottleneck @ file:///home/conda/feedstock_root/build_artifacts/bottleneck_1656803757560/work
certifi==2022.12.7
charset-normalizer==2.1.1
click==8.1.3
cloudpickle==2.2.1
ctxcore==0.2.0
cycler==0.11.0
cytoolz==0.12.1
dask==2022.2.0
debugpy==1.6.6
decorator==5.1.1
dill==0.3.6
distributed==2022.2.0
entrypoints==0.4
fonttools==4.38.0
frozendict==2.3.4
frozenlist==1.3.3
fsspec==2023.1.0
h5py==3.8.0
HeapDict==1.0.1
idna==3.4
importlib-metadata==6.0.0
interlap==0.2.7
ipykernel==6.16.2
ipython==7.34.0
jedi==0.18.2
Jinja2==3.1.2
joblib==1.2.0
jupyter_client==7.4.9
jupyter_core==4.12.0
kiwisolver==1.4.4
llvmlite==0.39.1
locket==1.0.0
loompy==3.0.7
MarkupSafe==2.1.2
matplotlib==3.5.3
matplotlib-inline==0.1.6
msgpack==1.0.4
multidict==6.0.4
multiprocessing-on-dill==3.5.0a4
nest-asyncio==1.5.6
networkx==2.6.3
numba==0.56.4
numexpr==2.8.4
numpy==1.21.6
numpy-groupies==0.9.20
packaging @ file:///home/conda/feedstock_root/build_artifacts/packaging_1673482170163/work
pandas==1.3.5
parso==0.8.3
partd==1.3.0
pexpect==4.8.0
pickleshare==0.7.5
Pillow==9.4.0
prompt-toolkit==3.0.36
psutil==5.9.4
ptyprocess==0.7.0
pyarrow==0.16.0
Pygments==2.14.0
pynndescent==0.5.8
pyparsing==3.0.9
pyscenic==0.11.1
python-dateutil @ file:///home/conda/feedstock_root/build_artifacts/python-dateutil_1626286286081/work
pytz @ file:///home/conda/feedstock_root/build_artifacts/pytz_1673864280276/work
PyYAML==6.0
pyzmq==25.0.0
requests==2.28.2
scikit-learn==1.0.2
scipy==1.7.3
seaborn==0.12.2
six @ file:///home/conda/feedstock_root/build_artifacts/six_1620240208055/work
sortedcontainers==2.4.0
tblib==1.7.0
threadpoolctl==3.1.0
toolz==0.12.0
tornado==6.2
tqdm==4.64.1
traitlets==5.8.1
typing_extensions==4.4.0
umap-learn==0.5.3
urllib3==1.26.14
wcwidth==0.2.6
yarl==1.8.2
zict==2.2.0
zipp==3.11.0

[carlos-a-enriquez]

Have you tried using the most recent version of pyscenic? I would also try checking whether the line dbs = [RankingDatabase(fname=fname, name=name(fname)) for fname in db_fnames] ran correctly. Inspect the dbs object.

[HaiyunChen0]

hello, I encounter the same problem, I would like to ask, have you solved this problem and how did you solve it?